Anticorps Polyclonal de lapin anti-IGSF8/CD316

IGSF8/CD316 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 14387-1-AP

Synonymes

CD316, CD81 partner 3, CD81P3, EWI 2, EWI2, IGSF8, KCT 4, KCT4, LIR D1, PGRL

---

Galerie de données de validation

• 

  WB analysis of K-562 using 14387-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14387-1-AP (IGSF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14387-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14387-1-AP (IGSF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14387-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14387-1-AP (IGSF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14387-1-AP

  IP result of anti-IGSF8/CD316 (IP:14387-1-AP, 3ug; Detection:14387-1-AP 1:500) with mouse brain tissue lysate 5000ug.

---

Applications testées

• Résultats positifs en WB: cellules K-562, cellules HEK-293, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

14387-1-AP cible IGSF8/CD316 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IGSF8/CD316 Protéine recombinante Ag5846
• Nom complet: immunoglobulin superfamily, member 8
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC053881
• Symbole du gène: IGSF8
• Identification du gène (NCBI): 93185
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IGSF8 is also known as CD316, PGRL (PG regulatory-like protein), KAI/CD82-associated surface molecule, and CD81-binding partner 3 (CD81P3). IGSF8 contains four immunoglobulin domains, a transmembrane region, and a short cytoplasmic tail that does not bear any signature motif for signal transduction. IGSF8 which is abundantly expressed in olfactory sensory neuron (OSN) axons and their developing synapses. (PMID: 17785435, PMID: 22687584)

Publications

Species	Application	Title
rat	WB	Mol Ther Nucleic Acids; Exosomal miR-365a-5p derived from HUC-MSCs regulates osteogenesis in GIONFH through the Hippo signaling pathway.; Authors - Ming-Jie Kuang
Mouse	WB	Int J Biol Sci; Exosomes derived from Wharton's jelly of human umbilical cord mesenchymal stem cells reduce osteocyte apoptosis in glucocorticoid-induced osteonecrosis of the femoral head in rats via the miR-21-PTEN-AKT signalling pathway.; Authors - Ming-Jie Kuang
rat	WB	Biochem Biophys Res Commun; Human umbilical cord mesenchymal stem cell-derived exosomes act via the miR-1263/Mob1/Hippo signaling pathway to prevent apoptosis in disuse osteoporosis.; Authors - Bao-Cheng Yang
Human	WB,IHC	Cell Death Dis; KLF4-mediated upregulation of CD9 and CD81 suppresses hepatocellular carcinoma development via JNK signaling.; Authors - Yandong Li

{{ptg:RelatedPrimaryAntibodies}}