Anticorps Polyclonal de lapin anti-IHH

IHH Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(11)

Avis (1)

N° de cat : 13388-1-AP

Synonymes

BDA1, EC:3.1.-.-, HHG 2, HHG2, HHG-2

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Galerie de données de validation

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  WB analysis of human kidney using 13388-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13388-1-AP (IHH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 13388-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13388-1-AP (IHH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 13388-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13388-1-AP (IHH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 13388-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13388-1-AP (IHH Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 13388-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13388-1-AP (IHH Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 13388-1-AP

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 13388-1-AP (IHH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13388-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13388-1-AP (IHH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13388-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13388-1-AP (IHH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13388-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13388-1-AP (IHH antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 13388-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13388-1-AP (IHH antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu rénal humain, cellules HEK-293, tissu cardiaque humain
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer de l'estomac humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 5 publications below
• IF: See 4 publications below

Informations sur le produit

13388-1-AP cible IHH dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IHH Protéine recombinante Ag4046
• Nom complet: Indian hedgehog homolog (Drosophila)
• Masse moléculaire calculée: 411 aa, 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC034757
• Symbole du gène: IHH
• Identification du gène (NCBI): 3549
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.; Authors - Rongxuan Zhu
mouse	IF	Cell Mol Life Sci; Adamts17 is involved in skeletogenesis through modulation of BMP-Smad1/5/8 pathway.; Authors - Takeshi Oichi
human	IHC	Mol Ther; Intra-articular Delivery of Antago-miR-483-5p Inhibits Osteoarthritis by Modulating Matrilin 3 and Tissue Inhibitor of Metalloproteinase 2.; Authors - Hua Wang
mouse	IHC	Osteoarthritis Cartilage; mTORC1 activation downregulates FGFR3 and PTH/PTHrP receptor in articular chondrocytes to initiate osteoarthritis.; Authors - H Zhang
mouse	WB,IHC	Int J Mol Sci; Inhibition of Ihh Reverses Temporomandibular Joint Osteoarthritis via a PTH1R Signaling Dependent Mechanism.; Authors - Hongxu Yang
mouse	IHC	Dev Biol; DDB1 promotes the proliferation and hypertrophy of chondrocytes during mouse skeleton development.; Authors - Lianzheng Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hongxue (Verified Customer) (05-13-2022)

WB works well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse liver

IHH Antibody Western Blot validation (1:1000 dilution) in Mouse liver (Cat no:13388-1-AP)

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