Anticorps Recombinant de lapin anti-IKKB

IKKB Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250538F11

N° de cat : 81347-2-RR

Synonymes

IKBKB, EC:2.7.11.1, EC:2.7.11.10, I kappa B kinase 2, I kappa B kinase beta

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Galerie de données de validation

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  WB analysis using 81347-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81347-2-RR (IKKB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of THP-1 using 81347-2-RR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using IKKB antibody (81347-2-RR, Clone: 250538F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 81347-2-RR

  Biolayer interferometry (BLl) kinetic assays of 81347-2-RR against Human IKKB were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules PC-12, cellules THP-1
• Résultats positifs en IF/ICC: cellules THP-1,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

81347-2-RR cible IKKB dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IKKB Protéine recombinante Ag8191
• Nom complet: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
• Masse moléculaire calculée: 756aa,81 kDa; 256aa,29 kDa
• Poids moléculaire observé: 80-85 kDa
• Numéro d’acquisition GenBank: BC006231
• Symbole du gène: IKBKB
• Identification du gène (NCBI): 3551
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IKBKB, also named as IKKB, IKK2, NFKBIKB and IKK-B, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and I-kappa-B kinase subfamily. IKBKB is a Serine kinase that plays an essential role in the NF-kappa-B signaling pathway. It acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. In addition to the NF-kappa-B inhibitors, IKBKB phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. It also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, IKBKB acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation.

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