Anticorps Monoclonal anti-IKBKG

IKBKG Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F2E2

N° de cat : 66460-1-PBS

Synonymes

AMCBX1, FIP3, FIP-3, Fip3p, I-kappa-B kinase subunit gamma

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Galerie de données de validation

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  WB analysis using 66460-1-Ig (same clone as 66460-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66460-1-Ig (IKBKG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66460-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66460-1-Ig (same clone as 66460-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66460-1-Ig (IKBKG antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66460-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66460-1-Ig (same clone as 66460-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66460-1-Ig (IKBKG antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66460-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66460-1-Ig (same clone as 66460-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IKBKG antibody (66460-1-Ig, Clone: 1F2E2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66460-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66460-1-PBS cible IKBKG dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IKBKG Protéine recombinante Ag13358
• Nom complet: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC012114
• Symbole du gène: IKBKG
• Identification du gène (NCBI): 8517
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IKBKG, also named as FIP3, NEMO, IKKAP1 and IKKG, is specifically phosphorylate serine or threonine residues that are followed by a proline residue. IKBKG is regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways.

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