Anticorps Recombinant de lapin anti-IKKA

IKKA Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241579A2

N° de cat : 84372-4-PBS

Synonymes

CHUK, IKK A, IKK alpha, IKK-1, IKK-alpha

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Galerie de données de validation

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  WB analysis using 84372-4-RR (same clone as 84372-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84372-4-RR (IKKA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84372-4-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 84372-4-RR (same clone as 84372-4-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug IKKA Recombinant antibody (84372-4-RR, Clone:241579A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84372-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84372-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84372-4-RR against Human IKKA were performed. The affinity constant is 19.5 nM.

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Informations sur le produit

84372-4-PBS cible IKKA dans les applications de WB, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IKKA Protéine recombinante Ag26573
• Nom complet: conserved helix-loop-helix ubiquitous kinase
• Masse moléculaire calculée: 85 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: NM_001278
• Symbole du gène: IKK Alpha
• Identification du gène (NCBI): 1147
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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