Anticorps Recombinant de lapin anti-IKKA

IKKA Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241579D12

N° de cat : 84372-5-PBS

Synonymes

CHUK, IKK A, IKK alpha, IKK-1, IKK-alpha

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Galerie de données de validation

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  IF Staining of HCT 116 using 84372-5-RR (same clone as 84372-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using IKKA antibody (84372-5-RR, Clone: 241579D12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84372-5-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 84372-5-RR (same clone as 84372-5-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug IKKA Recombinant Antibody (84372-5-RR, Clone:241579D12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84372-5-PBS in a different storage buffer formulation.

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Informations sur le produit

84372-5-PBS cible IKKA dans les applications de IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IKKA Protéine recombinante Ag26573
• Nom complet: conserved helix-loop-helix ubiquitous kinase
• Masse moléculaire calculée: 85 kDa
• Numéro d’acquisition GenBank: NM_001278
• Symbole du gène: IKK Alpha
• Identification du gène (NCBI): 1147
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NF‐κB is regulated by phosphorylation of one of its cytoplasmic inhibitors, IκB, mediated by IκB kinases (IKK), allowing NF‐κB nuclear translocation and transcriptional activation. IKKα‐IKKβ heterodimer complexes regulate the DNA binding proteins implicated in the canonical NF‐κB pathway, including p50‐p65, while IKKα‐homodimers regulate p52‐RELB dimers implicated in the alternative NF‐κB pathway. (PMID: 34187082)

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