Anticorps Monoclonal anti-IKZF1

IKZF1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

1D6B5

N° de cat : 66966-1-Ig

Synonymes

DNA binding protein Ikaros, hIk 1, Hs.54452, IK1, IKAROS, IKZF1, LYF1, PRO0758, ZNFN1A1

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Galerie de données de validation

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  WB analysis using 66966-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit spleen using 66966-1-Ig

  rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig thymus using 66966-1-Ig

  pig thymus tissue were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66966-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 66966-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66966-1-Ig (IKZF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules Daudi, cellules Jurkat, cellules MOLT-4, cellules Ramos, cellules Sp2/0, tissu de thymus de porc, tissu splénique de lapin

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

66966-1-Ig cible IKZF1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, souris

• Réactivité: Humain, Lapin, porc, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IKZF1 Protéine recombinante Ag28637
• Nom complet: IKAROS family zinc finger 1 (Ikaros)
• Masse moléculaire calculée: 477 aa, 53 kDa
• Poids moléculaire observé: 55-65 kDa
• Numéro d’acquisition GenBank: BC018349
• Symbole du gène: IKZF1
• Identification du gène (NCBI): 10320
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IKZF1, also known as DNA-binding protein Ikaros, Belongs to the Ikaros C2H2-type zinc-finger protein family. The N-terminal zinc-fingers 2 and 3 are required for DNA binding as well as for targeting IKFZ1 to pericentromeric heterochromatin. The C-terminal zinc-finger domain is required for dimerization. IKZF is abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. IKZF1 is localized in nucleus. IKZF1 as a transcription regulator of hematopoietic cell differentiation binds gamma-satellite DNA and plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. IKZF1 is a repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. IKZF1 exists some isofroms with MV 58KDa, 48KDa, 48KDa, 43KDa, 41KDa, 32KDa and 53KDa, respectively.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; Transcriptional Regulation of PIK3CD and PIKFYVE in T-Cell Acute Lymphoblastic Leukemia by IKAROS and Protein Kinase CK2.; Authors - Elanora Dovat ; KD Validated