Anticorps Monoclonal anti-IKZF1

• Phare
• Validé par KD/KO

IKZF1 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D6B5

N° de cat : 66966-1-PBS

Synonymes

DNA binding protein Ikaros, DNA-binding protein Ikaros, hIk 1, Hs.54452, IK1

---

Galerie de données de validation

• 

  WB analysis using 66966-1-Ig (same clone as 66966-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit spleen using 66966-1-Ig (same clone as 66966-1-PBS)

  rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig thymus using 66966-1-Ig (same clone as 66966-1-PBS)

  pig thymus tissue were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66966-1-Ig (same clone as 66966-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MOLT-4 using 66966-1-Ig (same clone as 66966-1-PBS)

  WB result of IKZF1 antibody (66966-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IKZF1 transfected MOLT-4 cells. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 66966-1-Ig (same clone as 66966-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66966-1-Ig (IKZF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66966-1-PBS cible IKZF1 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, souris

• Réactivité: Humain, Lapin, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IKZF1 Protéine recombinante Ag28637
• Nom complet: IKAROS family zinc finger 1 (Ikaros)
• Masse moléculaire calculée: 477 aa, 53 kDa
• Poids moléculaire observé: 55-65 kDa
• Numéro d’acquisition GenBank: BC018349
• Symbole du gène: IKZF1
• Identification du gène (NCBI): 10320
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IKZF1, also known as DNA-binding protein Ikaros, Belongs to the Ikaros C2H2-type zinc-finger protein family. The N-terminal zinc-fingers 2 and 3 are required for DNA binding as well as for targeting IKFZ1 to pericentromeric heterochromatin. The C-terminal zinc-finger domain is required for dimerization. IKZF is abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. IKZF1 is localized in nucleus. IKZF1 as a transcription regulator of hematopoietic cell differentiation binds gamma-satellite DNA and plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. IKZF1 is a repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. IKZF1 exists some isofroms with MV 58KDa, 48KDa, 48KDa, 43KDa, 41KDa, 32KDa and 53KDa, respectively.

{{ptg:RelatedPrimaryAntibodies}}