Anticorps Polyclonal de lapin anti-Aiolos/IKZF3

Aiolos/IKZF3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 19055-1-PBS

Synonymes

IKZF3, ZNFN1A3, Zinc finger protein Aiolos, IKZF 3, AIOLOS

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Galerie de données de validation

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  WB analysis of HepG2 using 19055-1-AP (same clone as 19055-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19055-1-AP (IKZF3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19055-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 19055-1-AP (same clone as 19055-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19055-1-AP (IKZF3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19055-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 19055-1-AP (same clone as 19055-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19055-1-AP (IKZF3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19055-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 19055-1-AP (same clone as 19055-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 19055-1-AP (IKZF3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19055-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 19055-1-AP (same clone as 19055-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 19055-1-AP (IKZF3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19055-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 19055-1-AP (same clone as 19055-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using IKZF3 antibody 19055-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 19055-1-PBS in a different storage buffer formulation.

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Informations sur le produit

19055-1-PBS cible Aiolos/IKZF3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Aiolos/IKZF3 Protéine recombinante Ag5537
• Nom complet: IKAROS family zinc finger 3 (Aiolos)
• Masse moléculaire calculée: 509 aa, 58 kDa
• Poids moléculaire observé: 58-70 kDa
• Numéro d’acquisition GenBank: BC032707
• Symbole du gène: IKZF3
• Identification du gène (NCBI): 22806
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IKZF3, also named as Zinc finger protein Aiolos or ZNFN1A3, is a 509 amino acid protein, which contains 6 C2H2-type zinc fingers and belongs to the Ikaros C2H2-type zinc-finger protein family. IKZF3 localizes in the nucleus and cytoplasm. IKZF3 can form homodimer, and heterodimer with other IKAROS family members. IKZF3 is expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus. IKZF3 as a transcription factor plays an important role in the regulation of lymphocyte differentiation. The calculated molecular weight of IKZF3 is 58 kDa, but the modified IKZF3 protein is about 65-70 kDa.

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