Anticorps Recombinant de lapin anti-IKZF4

IKZF4 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240357D5

N° de cat : 83471-5-PBS

Synonymes

ZNFN1A4, Zinc finger protein Eos, KIAA1782, EOS, 240357D5

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Galerie de données de validation

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  WB analysis of K-562 using 83471-5-RR (same clone as 83471-5-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 83471-5-RR (IKZF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83471-5-PBS in a different storage buffer formulation.

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  WB analysis using 83471-5-RR (same clone as 83471-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83471-5-RR (IKZF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83471-5-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 83471-5-RR (same clone as 83471-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using IKZF4 antibody (83471-5-RR, Clone: 240357D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83471-5-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 83471-5-RR (same clone as 83471-5-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug IKZF4 Recombinant antibody (83471-5-RR, Clone:240357D5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83471-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83471-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83471-5-RR against Human IKZF4 were performed. The affinity constant is 21.3 nM.

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Informations sur le produit

83471-5-PBS cible IKZF4 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IKZF4 Protéine recombinante Ag34767
• Nom complet: IKAROS family zinc finger 4 (Eos)
• Numéro d’acquisition GenBank: BC160169
• Symbole du gène: IKZF4
• Identification du gène (NCBI): 64375
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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