Anticorps Monoclonal anti-IL-1 beta

IL-1 beta Monoclonal Antibody for FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2A7C6

N° de cat : 65276-1-PBS

Synonymes

2A7C6, Catabolin, IL 1, IL 1 beta, IL1 beta

---

Galerie de données de validation

• 

  FC experiment of human PBMCs using 65276-1-Ig (same clone as 65276-1-PBS)

  1X10^6 LPS and Brefeldin A treated human PBMCs were surface stained with Anti-Human HLA-DR then intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-1β (CL488-65276, Clone:2A7C6) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65276-1-Ig. This data was developed using the same antibody clone with 65276-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65276-1-Ig (same clone as 65276-1-PBS)

  1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-1β (CL488-65276, Clone:2A7C6) (red) or Mouse IgG1 Isotype Control (blue), or unactivated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-1β (CL488-65276, Clone:2A7C6) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65276-1-Ig. This data was developed using the same antibody clone with 65276-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 65276-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 65276-1-Ig against IL-1 beta were performed. The affinity constant is 102 nM.

---

Informations sur le produit

65276-1-PBS cible IL-1 beta dans les applications de FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-1 beta Protéine recombinante HZ-1164
• Nom complet: interleukin 1, beta
• Masse moléculaire calculée: 269 aa, 31 kDa
• Numéro d’acquisition GenBank: BC008678
• Symbole du gène: IL1B
• Identification du gène (NCBI): 3553
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. Interleukin 1β (IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. (PMID: 8630372; 12401481; 23704929; 24618930)

{{ptg:RelatedPrimaryAntibodies}}