Anticorps Monoclonal anti-IL-1 beta

• Phare
• Validé par KD/KO

IL-1 beta Monoclonal Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2A1B4

N° de cat : 66737-1-PBS

Synonymes

2A1B4, Catabolin, IL 1, IL 1 beta, IL1 beta

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Galerie de données de validation

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  WB analysis using 66737-1-Ig (same clone as 66737-1-PBS)

  Untreated, LPS and Brefeldin A-treated THP-1 cells were subjected to SDS PAGE followed by western blot with 66737-1-Ig (IL-1 beta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 66737-1-Ig (same clone as 66737-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66737-1-Ig (IL-1 Beta antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

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  WB analysis of U-87 MG using 66737-1-Ig (same clone as 66737-1-PBS)

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 66737-1-Ig (IL-1 Beta antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP80001-6

  Sandwich ELISA standard curve of MP80001-6, IL-1 beta Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 82696-8-PBS. Detection antibody: 66737-1-PBS. Standard:HZ-1164. Range: 15.6-1000 pg/mL.

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  IHC staining of human lung cancer using 66737-1-Ig (same clone as 66737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66737-1-Ig (same clone as 66737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66737-1-Ig (same clone as 66737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66737-1-Ig (same clone as 66737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66737-1-Ig (same clone as 66737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 66737-1-Ig (same clone as 66737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 66737-1-Ig (same clone as 66737-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed LPS treated THP-1 cells using IL-1 beta antibody (66737-1-Ig, Clone: 2A1B4 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66737-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 66737-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 66737-1-Ig against Human IL1B were performed. The affinity constant is 5.03 nM.

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Informations sur le produit

66737-1-PBS cible IL-1 beta dans les applications de WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-1 beta Protéine recombinante Ag26030
• Nom complet: interleukin 1, beta
• Masse moléculaire calculée: 269 aa, 31 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC008678
• Symbole du gène: IL1B
• Identification du gène (NCBI): 3553
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.

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