Anticorps Recombinant de lapin anti-IL-1 beta

IL-1 beta Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

240902D6

N° de cat : 82696-15-RR

Synonymes

IL1- beta, IL1 beta, IL 1 beta, IL 1, Catabolin

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Galerie de données de validation

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  WB analysis of THP-1 using 82696-15-RR

  LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 82696-15-RR (IL-1 beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 82696-15-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82696-15-RR (IL1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of THP-1 using 82696-15-RR

  Immunofluorescent analysis of (4% PFA) fixed LPS and Brefeldin A treated THP-1 cells using IL-1 beta antibody (82696-15-RR, Clone: 240902D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 82696-15-RR

  Biolayer interferometry (BLl) kinetic assays of 82696-15-RR against Human IL-1 beta were performed. The affinity constant is 2.09 nM.

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Applications testées

• Résultats positifs en WB: LPS et à la bréfeldine A, cellules A431, cellules HT-1080, cellules MG U-87
• Résultats positifs en IF/ICC: LPS et à la bréfeldine A,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

82696-15-RR cible IL-1 beta dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 1, beta
• Masse moléculaire calculée: 269 aa, 31 kDa
• Poids moléculaire observé: 30-35 kDa
• Numéro d’acquisition GenBank: BC008678
• Symbole du gène: IL1B
• Identification du gène (NCBI): 3553
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.

Publications

Species	Application	Title
human	WB	Food Sci Nutr; Resveratrol Promotes Wound Healing by Enhancing Angiogenesis via Inhibition of Ferroptosis; Authors - Yujie Pan

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