Anticorps Recombinant de lapin anti-IL-1 alpha

IL-1 alpha Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

FITC Plus Fluorescent Dye

CloneNo.

240431G10

N° de cat : FITC-98052

Synonymes

Il1a, IL1-alpha, 240431G10, Il 1a, IL1 alpha

---

Galerie de données de validation

• 

  FC experiment of RAW 264.7 using FITC-98052

  1x10^6 untreated or LPS-treated RAW 264.7 cells were intracellularly stained with 0.25 ug FITC Plus Anti-Mouse IL-1 alpha Rabbit RecAb (FITC-98052, Clone: 240431G10). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• 

  FC experiment of RAW 264.7 using FITC-98052

  1x10^6 LPS-treated RAW 264.7 cells were intracellularly stained with 0.25 ug FITC Plus Anti-Mouse IL-1 alpha Rabbit RecAb (FITC-98052, Clone: 240431G10) (red) or 0.25 ug FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9) (blue). 1x10^6 untreated RAW 264.7 cells were intracellularly stained with 0.25 ug FITC Plus Anti-Mouse IL-1 alpha Rabbit RecAb (FITC-98052, Clone: 240431G10) (black, dashed). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

---

Applications testées

• Résultats positifs en FC (Intra): cellules RAW 264.7 traitées au LPS,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-98052 cible IL-1 alpha dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IL-1 alpha Protéine recombinante Eg0263
• Nom complet: interleukin 1 alpha
• Masse moléculaire calculée: 31kd
• Numéro d’acquisition GenBank: NM_010554
• Symbole du gène: Il1a
• Identification du gène (NCBI): 16175
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

IL-1 alpha is a dual function cytokine that can function both as a transcription factor and a damage-associated molecular pattern (DAMP) in innate immune responses. It is expressed by many cell types and its expression is increased when cells are exposed to growth factors, pro-inflammatory, and stress-related signals. In its pro-form, IL-1 alpha contains a nuclear localization signal that allows it to translocate to the nucleus and bind to DNA, resulting in either the increase or prohibition of pro-inflammatory cytokine expression. When cells are exposed to necrotic signals, mature IL-1 alpha is released from the cell lysates and triggers a strong pro-inflammatory response upon binding to its receptor on nearby cells. Due to its strongly pro-inflammatory nature, IL-1 alpha has been linked to tumor metastasis and lower median survival in several cancers (PMID: 27434011, 29247995).

{{ptg:RelatedPrimaryAntibodies}}