Anticorps Monoclonal anti-IL-1 beta

IL-1 beta Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

2A7C6

N° de cat : 65276-1-Ig

Synonymes

2A7C6, Catabolin, IL 1, IL 1 beta, IL1 beta

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Galerie de données de validation

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  FC experiment of human PBMCs using 65276-1-Ig

  1X10^6 LPS and Brefeldin A treated human PBMCs were surface stained with Anti-Human HLA-DR then intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-1β (CL488-65276, Clone:2A7C6) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65276-1-Ig.

• 

  FC experiment of human PBMCs using 65276-1-Ig

  1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-1β (CL488-65276, Clone:2A7C6) (red) or Mouse IgG1 Isotype Control (blue), or unactivated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-1β (CL488-65276, Clone:2A7C6) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65276-1-Ig.

• 

  Affinity and Kinetic Characterization of 65276-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 65276-1-Ig against IL-1 beta were performed. The affinity constant is 102 nM.

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Applications testées

• Résultats positifs en FC (Intra): LPS and Brefeldin A treated human PBMCs

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65276-1-Ig cible IL-1 beta dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-1 beta Protéine recombinante HZ-1164
• Nom complet: interleukin 1, beta
• Masse moléculaire calculée: 269 aa, 31 kDa
• Numéro d’acquisition GenBank: BC008678
• Symbole du gène: IL1B
• Identification du gène (NCBI): 3553
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. Interleukin 1β (IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. (PMID: 8630372; 12401481; 23704929; 24618930)

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