Anticorps Recombinant de lapin anti-IL-1 beta

IL-1 beta Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240902G6

N° de cat : 82696-17-PBS

Synonymes

240902G6, Catabolin, IL 1, IL 1 beta, IL1 beta

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Galerie de données de validation

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  WB analysis using 82696-17-RR (same clone as 82696-17-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82696-17-RR (IL-1 beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82696-17-PBS in a different storage buffer formulation.

• 

  WB analysis of THP-1 using 82696-17-RR (same clone as 82696-17-PBS)

  LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 82696-17-RR (IL-1 beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82696-17-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82696-17-RR

  Biolayer interferometry (BLl) kinetic assays of 82696-17-RR against Human IL-1 beta were performed. The affinity constant is 60.2 nM.

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Informations sur le produit

82696-17-PBS cible IL-1 beta dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IL-1 beta Protéine recombinante Eg0286
• Nom complet: interleukin 1, beta
• Masse moléculaire calculée: 269 aa, 31 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC008678
• Symbole du gène: IL1B
• Identification du gène (NCBI): 3553
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to the activation of numerous downstream targets.

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