Anticorps Recombinant de lapin anti-IL-1 beta

IL-1 beta Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241364D5

N° de cat : 84136-5-RR

Synonymes

IL 1beta, Il1b, 241364D5, IL 1 beta, Il 1b

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Galerie de données de validation

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  WB analysis of THP-1 using 84136-5-RR

  LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 84136-5-RR (IL-1 beta antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 84136-5-RR

  LPS and Brefeldin A treated RAW264.7 cells were subjected to SDS PAGE followed by western blot with 84136-5-RR (IL-1 beta antibody) at dilution of 1:4000, incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84136-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84136-5-RR against Mouse IL-1 beta were performed. The affinity constant is 13.2 pM.

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Applications testées

• Résultats positifs en WB: LPS et à la bréfeldine A, cellules RAW 264.7 traitées au LPS et à la bréfeldine A

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84136-5-RR cible IL-1 beta dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IL-1 beta Protéine recombinante Eg1577
• Nom complet: interleukin 1 beta
• Masse moléculaire calculée: 31kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: NM_008361.4
• Symbole du gène: IL-1 beta
• Identification du gène (NCBI): 16176
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets.

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