Anticorps Monoclonal anti-IL-10

• Phare
• Validé par KD/KO

IL-10 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

8E4B6

N° de cat : 60269-1-PBS

Synonymes

IL-10,IL10, 8E4B6, CSIF, IL 10, IL10

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Galerie de données de validation

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  WB analysis of Recombinant protein using 60269-1-Ig (same clone as 60269-1-PBS)

  Recombinant protein were subjected to SDS PAGE followed by western blot with 60269-1-Ig (IL-10 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60269-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60269-1-Ig (same clone as 60269-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60269-1-Ig (IL-10 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60269-1-PBS in a different storage buffer formulation.

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  IF Staining of MOLT-4 using 60269-1-Ig (same clone as 60269-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MOLT-4 cells using IL-10 antibody (60269-1-Ig, Clone: 8E4B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60269-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60269-1-PBS cible IL-10 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-10 Protéine recombinante Ag14870
• Nom complet: interleukin 10
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC104252
• Symbole du gène: IL-10
• Identification du gène (NCBI): 3586
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

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