Anticorps Monoclonal anti-IL-10

IL-10 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

7B8G3

N° de cat : 65286-1-Ig

Synonymes

IL-10,IL10, 7B8G3, CSIF, IL 10, IL10

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Galerie de données de validation

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  FC experiment of human PBMCs using 65286-1-Ig

  1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-10 (CL488-65286, Clone:7B8G3) or Mouse IgG2b Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65286-1-Ig.

• 

  FC experiment of human PBMCs using 65286-1-Ig

  1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-10 (CL488-65286, Clone:7B8G3) (red) or Mouse IgG2b Isotype Control (blue), or unactivated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-10 (CL488-65286, Clone:7B8G3) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65286-1-Ig.

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Applications testées

• Résultats positifs en cytométrie: LPS and Brefeldin A treated human PBMCs,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65286-1-Ig cible IL-10 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-10 Protéine recombinante HZ-1145
• Nom complet: interleukin 10
• Masse moléculaire calculée: 21 kDa
• Numéro d’acquisition GenBank: BC104252
• Symbole du gène: IL-10
• Identification du gène (NCBI): 3586
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

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