Anticorps Recombinant de lapin anti-IL-10

IL-10 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(7)

CloneNo.

240430G12

N° de cat : 82191-3-RR

Synonymes

Il10, IL-10,IL10, 240430G12, CSIF, Il 10

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Galerie de données de validation

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  WB analysis using 82191-3-RR

  Untreated, PMA, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 82191-3-RR (Il10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 4 publications below

Informations sur le produit

82191-3-RR cible IL-10 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IL-10 Protéine recombinante Eg0026
• Nom complet: interleukin 10
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: NM_010548
• Symbole du gène: Il10
• Identification du gène (NCBI): 16153
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IL-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

Publications

Species	Application	Title
mouse	IHC	Bioeng Transl Med; Macrophages overexpressing interleukin-10 target and prevent atherosclerosis: Regression of plaque formation and reduction in necrotic core; Authors - Mingyi Wang
mouse	WB	Front Immunol; Acute hypoxia modulate macrophage phenotype accompanied with transcriptome re-programming and metabolic re-modeling; Authors - Binda Sun
mouse	IHC	Inflamm Res; Luteolin modulates liver macrophage subtype polarization and play protective role in sepsis induced acute hepatic injury; Authors - Liangyong Deng
mouse	WB	Apoptosis; Itaconate promotes mitophagy to inhibit neuronal ferroptosis after subarachnoid hemorrhage; Authors - Guijun Wang
rat	IHC	Adv Healthc Mater; Extracellular Vesicles from Adipose-Derived Mesenchymal Stem Cells Combined with PEG Hydrogel Alleviate Maternal Simulated Birth Injury in a Rat Model; Authors - Qing Wang
rat	IHC	J Transl Med; Fluorinated hydroxyapatite conditions a favorable osteo-immune microenvironment via triggering metabolic shift from glycolysis to oxidative phosphorylation; Authors - Kaidi Chen

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