Anticorps Monoclonal anti-IL-10

IL-10 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

7B8G3

N° de cat : CL647-65286

Synonymes

CSIF, IL 10, IL10, IL10A, interleukin 10, TGIF



Applications testées

Résultats positifs en cytométrieLPS and Brefeldin A treated human PBMCs,

Dilution recommandée

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65286 cible IL-10 dans les applications de FC et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG2b
Clonalité Monoclonal
Type Anticorps
Immunogène IL-10 Protéine recombinante HZ-1145
Nom complet interleukin 10
Masse moléculaire calculée 21 kDa
Numéro d’acquisition GenBankBC104252
Symbole du gène IL10
Identification du gène (NCBI) 3586
Conjugaison CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Forme Liquide
Méthode de purification Purification par affinité
Tampon de stockage PBS with 0.09% sodium azide and 0.5% BSA.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

Protocole

Product Specific Protocols
FC protocol for CL Plus 647 IL-10 antibody CL647-65286Download protocol
Standard Protocols
Click here to view our Standard Protocols