Anticorps Recombinant de lapin anti-IL-10RB

IL-10RB Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

242302D2

N° de cat : CL647-98292

Synonymes

IL10RB, 242302D2, CDW210B, CRF2 4, CRF2-4

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Galerie de données de validation

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  FC experiment of human PBMCs using CL647-98292

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody (CL488-65560, Clone: L243), and 5 ul CoraLite® Plus 647 Anti-Human IL-10RB Rabbit RecAb (CL647-98292, Clone: 242302D2) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using CL647-98292

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human IL-10RB Rabbit RecAb (CL647-98292, Clone:242302D2) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-98292 cible IL-10RB dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IL-10RB Protéine recombinante Eg3446
• Nom complet: interleukin 10 receptor, beta
• Masse moléculaire calculée: 37 kDa
• Numéro d’acquisition GenBank: BC001903
• Symbole du gène: IL10RB
• Identification du gène (NCBI): 3588
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Interleukin-10 (IL-10) is an anti-inflammatory cytokine that plays a critical role in maintaining immune system balance. IL-10 signals through a receptor complex consisting of two molecules of the IL-10R alpha-chain (IL-10RA) and two molecules of the accessory IL-10R beta-chain (IL-10RB) (PMID: 22236434). IL-10RA and IL-10RB are members of the type II cytokine receptor family. IL-10RB, also known as IL-10R2 or CDw210b, is the signaling subunit of the IL-10R complex and is constitutively expressed in a variety of cell types (PMID: 24507158). Binding of IL-10 to the IL-10R complex leads to JAK1- and TYK2-mediated phosphorylation of STAT3 (PMID: 11244051; 10433356). IL-10RB is also shared by several other cytokines, including IL22, IL-26, IFN-γ, IL-28A/B and IL29 (PMID: 22236434).

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