Anticorps Monoclonal anti-IL-12A/IL-12 p35

IL-12A/IL-12 p35 Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

1E12H6

N° de cat : 65277-1-PBS

Synonymes

IL-12, 1E12H6, CLMF, CLMF p35, IL 12 subunit p35

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Galerie de données de validation

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  FC experiment of human PBMCs using 65277-1-Ig (same clone as 65277-1-PBS)

  1X10^6 LPS and Brefeldin A treated human PBMCs were surface stained with CoraLite ® Plus 750 Anti-Human HLA-DR then intracellularly stained with 0.2 ug CoraLite ® Plus 488 Anti-Human IL-12 (CL488-65277, Clone:1E12H6) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65277-1-Ig. This data was developed using the same antibody clone with 65277-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65277-1-Ig (same clone as 65277-1-PBS)

  1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-12 (CL488-65277, Clone:1E12H6) (red) or Mouse IgG1 Isotype Control (blue), or unstimulated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-12 (CL488-65277, Clone:1E12H6) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65277-1-Ig. This data was developed using the same antibody clone with 65277-1-PBS in a different storage buffer formulation.

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Informations sur le produit

65277-1-PBS cible IL-12A/IL-12 p35 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-12A/IL-12 p35 Protéine recombinante HZ-1256
• Nom complet: interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
• Masse moléculaire calculée: 25 kDa
• Numéro d’acquisition GenBank: BC104982
• Symbole du gène: IL-12A
• Identification du gène (NCBI): 3592
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: -20°C. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les

Informations générales

IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells (PMID: 24821971; 7612223).

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