Anticorps Recombinant de lapin anti-IL-12 p70

IL-12 p70 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

230037B7

N° de cat : 82878-2-RR

Synonymes

Il 12b, Il 12p40, IL-12 P35, IL-12 p70, Il12b, Il12p40, interleukin 12b, p40

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Galerie de données de validation

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  WB analysis of Recombinant protein using 82878-2-RR

  Recombinant protein Eg0742 (IL-12 p70) were subjected to SDS PAGE followed by western blot with 82878-2-RR (IL-12 p70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 82878-2-RR

  Recombinant protein Eg0742 (IL-12 p70) and Recombinant protein Eg1821 (IL-12A p35)were subjected to SDS PAGE followed by western blot with 82878-2-RR (IL-12 p70 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 82878-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82878-2-RR against Mouse IL-12 p70 were performed. The affinity constant is 7.37 nM.

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Applications testées

• Résultats positifs en WB: Protéine recombinante,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82878-2-RR cible IL-12 p70 dans les applications de WB, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: IL-12 p70
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 70 kDa
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-12 (IL-12), is a cytokine that is secreted by activated phagocytes and dendritic cells and that induces interferon-γproduction by natural-killer and T lymphocytes. IL-12 is a 75 kDa heterodimer composed of a 35 kDa subunit (IL-12A p35) and a 40 kDa subunit (IL-12B p40) that is secreted by a wide variety of antigen presenting cells (APCs), including phagocytes, B cells and Langerhans cells.

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