Anticorps Recombinant de lapin anti-IL-13

IL-13 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240771B9

N° de cat : 83935-1-PBS

Synonymes

Il13, interleukin 13, 240771B9

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Galerie de données de validation

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  WB analysis of EL-4 using 83935-1-RR (same clone as 83935-1-PBS)

  Untreated EL-4 and EL-4 (treated with 50ng/mL PMA and Ionomycin 500ng/mL for 6h, replaced the culture medium with Brefeldin A for 18 hours) cells were subjected to SDS PAGE followed by western blot with 83935-1-RR (IL-13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83935-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83935-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83935-1-RR against Rat IL-13 were performed. The affinity constant is 0.419 nM.

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Informations sur le produit

83935-1-PBS cible IL-13 dans les applications de WB, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 13
• Masse moléculaire calculée: 14kDa
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: NM_053828.1
• Symbole du gène: Il13
• Identification du gène (NCBI): 116553
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Rat IL-13 is a kind of cytokines, which plays important roles in allergic inflammation and immune response to parasite infection. (PubMed:9366558). Rat IL-13 displays the capacity to antagonize Th1-driven proinflammatory immune response and downregulates synthesis of many proinflammatory cytokines including IL1, IL6, IL10, IL12 and TNF-alpha through a mechanism that partially involves suppression of NF-kappa-B (PubMed:9366558).

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