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Anticorps Polyclonal de lapin anti-IL-13Ra1/CD213a1

IL-13Ra1/CD213a1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 27916-1-AP

Synonymes

IL13RA1, Cancer/testis antigen 19, CD213A1, CT19, IL 13 receptor subunit alpha 1

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using IL-13Rα1 Polyclonal antibody (27916-1-AP)

    WB analysis using 27916-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27916-1-AP (IL-13RA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Applications testées

Résultats positifs en WBcellules HT-29, cellules Jurkat, cellules Raji, tissu de muscle squelettique de souris

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

27916-1-AP cible IL-13Ra1/CD213a1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène IL-13Ra1/CD213a1 Protéine recombinante Ag25542
Nom complet interleukin 13 receptor, alpha 1
Masse moléculaire calculée 49 kDa
Poids moléculaire observé70 kDa
Numéro d’acquisition GenBankBC009960
Symbole du gène IL-13Rα1
Identification du gène (NCBI) 3597
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IL-13 is an immunoregulatory cytokine secreted predominantly by activated T-helper 2 (Th2) cells and performs multiple functions through a complex receptor system comprised of IL-4Rα and two IL-13 binding proteins, IL-13Rα1 and IL-13Rα2 (PMID: 12704343). IL-13Rα1 is a 65- to 70-kd glycosylated transmembrane protein (PMID: 10856136; 9723655). IL-13Rα1 is expressed on a wide range of cells including B cells, monocytes, macrophages, basophils, mast cells, and endothelial cells (PMID: 10856136; 31866859). IL-13 binding to the IL-4Rα/IL-13Rα1 complex leads to activation of JAK1, JAK2, and TYK2 and subsequent phosphorylation of STAT6 and STAT3 (PMID: 31866859).

Protocole

Product Specific Protocols
WB protocol for IL-13Ra1/CD213a1 antibody 27916-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Ciara (Verified Customer) (01-30-2024)

Positive staining in N2a cells

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: Neuro-2a mouse neuroblastoma cell line
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