Anticorps Recombinant de lapin anti-IL-15

IL-15 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

230285B3

N° de cat : 98036-1-RR

Synonymes

230285B3, AI503618, Il15, interleukin 15

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Galerie de données de validation

• 

  FC experiment of Mouse PBMCs using 98036-1-RR

  1x10^6 Mouse PBMCs were intracellularly stained with 0.25 ug Anti-Mouse IL-15 Rabbit Recombinant Antibody (98036-1-RR, Clone: 230285B3) (red) or Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

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  Affinity and Kinetic Characterization of 98036-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98036-1-RR against Mouse IL-15 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en FC (Intra): Mouse PBMCs

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98036-1-RR cible IL-15 dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IL-15 Protéine recombinante Eg0359
• Nom complet: interleukin 15
• Masse moléculaire calculée: 19 kDa
• Numéro d’acquisition GenBank: NM_001254747
• Symbole du gène: IL-15
• Identification du gène (NCBI): 16168
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

IL-15 is a 4-α-helix bundle cytokine playing a pivotal role in stimulation of both innate and adaptive immune cells. It is produced primarily by keratinocytes, skeletal muscle cells, monocytes, and CD4+ T cells. It is a member of the common gamma chain family. The members of the common gamma chain family include the IL-2, IL-4, IL-7, IL-9, and IL-21 and require binding to the common gamma chain receptor for activation. It can be used in growth and maintenance of T and NK cells. It is also shown to be used in proliferation and functional effect of T cells for adoptive cell therapy. It is a glycosylated protein and HumanKine IL-15 appear as 12.5-25 kDa bands (PMID: 24587813, 26627006, 27849617, 31250350)

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