Anticorps Recombinant de lapin anti-IL-15RA

IL-15RA Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

242689E1

N° de cat : 98380-3-RR

Synonymes

IL15RA, CD215, IL 15 receptor subunit alpha, IL 15R alpha, IL 15RA

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Galerie de données de validation

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  FC experiment of Human Monocytes using 98380-3-RR

  1x10^6 untreated or LPS and IFN-γ treated human monocytes were surface stained with 0.25 ug Anti-Human IL-15RA Rabbit RecAb (98380-3-RR, Clone:242689E1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were not fixed.

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  FC experiment of Human Monocytes using 98380-3-RR

  1x10^6 LPS and IFN-γ treated human PBMCs were surface stained with 0.25 ug Anti-Human IL15RA Rabbit RecAb (98380-3-RR, Clone: 242689E1) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human IL15RA Rabbit RecAb (98380-3-RR, Clone: 242689E1) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: LPS and IFN-γ treated human monocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98380-3-RR cible IL-15RA dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 15 receptor, alpha
• Masse moléculaire calculée: 231 aa, 24 kDa
• Numéro d’acquisition GenBank: BC107777
• Symbole du gène: IL-15RA
• Identification du gène (NCBI): 3601
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

Interleukin-15 (IL-15) is a pleiotropic cytokine that plays an important role in both innate and adaptive immunity. IL-15 is mainly produced by activated monocytes, macrophages and dendritic cells, and is structurally similar to IL-2 (PMID: 8178155; 12401478). These two cytokines share the same IL-2/15Rβ and common γ-chain receptor subunits. In addition, IL-2 and IL-15 have their own private α-chain receptor subunit, IL-2Rα and IL-15Rα, respectively (PMID: 7641685). The IL-15Rα chain is expressed by many cell types including monocytes, DCs, NK, T cells and fibroblasts. Several isoforms of IL-15Rα exist and are generated either by alternative splicing or by proteolytic cleavage (PMID: 10480910; 15265897).

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