Anticorps Recombinant de lapin anti-IL-15RA

IL-15RA Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

PE Fluorescent Dye

CloneNo.

242689E1

N° de cat : PE-98380-3

Synonymes

IL15RA, CD215, IL 15 receptor subunit alpha, IL 15R alpha, IL 15RA

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Galerie de données de validation

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  FC experiment of human PBMCs using PE-98380-3

  1x10^6 untreated or LPS and IFN-γ treated human PBMCs were surface stained with APC Anti-Human CD14 and 5 ul PE Anti-Human IL-15RA Rabbit RecAb (PE-98380-3, Clone:242689E1). Cells were not fixed.

• 

  FC experiment of human PBMCs using PE-98380-3

  1x10^6 LPS and IFN-γ treated human PBMCs were surface stained with 5 ul PE Anti-Human IL15RA Rabbit RecAb (PE-98380-3, Clone: 242689E1) (red) or PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul PE Anti-Human IL15RA Rabbit RecAb (PE-98380-3, Clone: 242689E1) (black, dashed). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: LPS and IFN-γ treated human PBMCs,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

PE-98380-3 cible IL-15RA dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 15 receptor, alpha
• Masse moléculaire calculée: 231 aa, 24 kDa
• Numéro d’acquisition GenBank: BC107777
• Symbole du gène: IL-15RA
• Identification du gène (NCBI): 3601
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Interleukin-15 (IL-15) is a pleiotropic cytokine that plays an important role in both innate and adaptive immunity. IL-15 is mainly produced by activated monocytes, macrophages and dendritic cells, and is structurally similar to IL-2 (PMID: 8178155; 12401478). These two cytokines share the same IL-2/15Rβ and common γ-chain receptor subunits. In addition, IL-2 and IL-15 have their own private α-chain receptor subunit, IL-2Rα and IL-15Rα, respectively (PMID: 7641685). The IL-15Rα chain is expressed by many cell types including monocytes, DCs, NK, T cells and fibroblasts. Several isoforms of IL-15Rα exist and are generated either by alternative splicing or by proteolytic cleavage (PMID: 10480910; 15265897).

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