Anticorps Recombinant de lapin anti-IL-16

IL-16 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250058G12

N° de cat : 86421-1-RR

Synonymes

Il16, Interleukin-16, Pro-interleukin-16

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Galerie de données de validation

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  WB analysis of RAW 264.7 using 86421-1-RR

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 86421-1-RR (Il16 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86421-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86421-1-RR (Il16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, tissu de thymus de rat, tissu de thymus de souris, tissu splénique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86421-1-RR cible IL-16 dans les applications de WB, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IL-16 Protéine recombinante Eg3127
• Nom complet: interleukin 16
• Masse moléculaire calculée: 141 kDa
• Poids moléculaire observé: 66-72 kDa
• Numéro d’acquisition GenBank: NM_010551.3
• Symbole du gène: Il16
• Identification du gène (NCBI): 16170
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IL-16 is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. It is mainly produced by T lymphocytes but also by other immune cells, neuronal cells, fibroblasts and epithelial cells. The signaling process of this cytokine is mediated by CD4. IL-16 induces chemotaxis of CD4+ cells such as lymphocytes, eosinophils, and dendritic cells by ligating CD4 directly at a site distinct from other ligands. Among its multiple functions, IL-16 is a T cell chemoattractant involved in T helper cell inflammatory responses and the regulation of both T cell growth, and responsiveness to regulatory cytokines. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Pro-IL-16 can be detected at about 80 kDa, and cleaved fragments can be deteced at between 40 and 75 kDa.

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