Anticorps Monoclonal anti-IL-17A

• Phare
• Validé par KD/KO

IL-17A Monoclonal Antibody for WB, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

1B3D5

N° de cat : 66148-1-PBS

Synonymes

IL 17, IL17, IL-17, IL17A, 1B3D5

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Galerie de données de validation

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  WB analysis using 66148-1-Ig (same clone as 66148-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66148-1-Ig (IL-17A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66148-1-Ig (same clone as 66148-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66148-1-Ig (IL-17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 66148-1-Ig (same clone as 66148-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 66148-1-Ig (IL-17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 66148-1-Ig (same clone as 66148-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66148-1-Ig (IL-17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66148-1-Ig (same clone as 66148-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-17A antibody (66148-1-Ig, Clone: 1B3D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 66148-1-Ig (same clone as 66148-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-17A antibody (66148-1-Ig, Clone: 1B3D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66148-1-PBS cible IL-17A dans les applications de WB, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-17A Protéine recombinante Ag3733
• Nom complet: interleukin 17A
• Masse moléculaire calculée: 155 aa, 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC067505
• Symbole du gène: IL-17A
• Identification du gène (NCBI): 3605
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IL17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.

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