Anticorps Recombinant de lapin anti-IL-17A

IL-17A Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230038B1

N° de cat : 82905-1-PBS

Synonymes

Il17a, Interleukin17A, interleukin 17A, Il17, IL 17A

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Galerie de données de validation

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  WB analysis of EL-4 using 82905-1-RR (same clone as 82905-1-PBS)

  PMA, Ionomycin and Brefeldin A treated EL-4 cells were subjected to SDS PAGE followed by western blot with 82905-1-RR (IL-17A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82905-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82905-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82905-1-RR against Mouse IL-17A were performed. The affinity constant is 7.59 nM.

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Informations sur le produit

82905-1-PBS cible IL-17A dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 17A
• Masse moléculaire calculée: 17 kDa
• Poids moléculaire observé: 14 kDa, 17 kDa
• Numéro d’acquisition GenBank: NM-010552
• Symbole du gène: Il17a
• Identification du gène (NCBI): 16171
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IL17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.

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