Anticorps Monoclonal anti-IL-17F

IL-17F Monoclonal Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

1C5C6

N° de cat : 68666-2-PBS

Synonymes

IL17F, Cytokine ML 1, Cytokine ML-1, IL 17F, interleukin 17F

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP50006-1

  Sandwich ELISA standard curve of MP50006-1, IL-17F Monoclonal Matched Antibody Pair, PBS Only. 68666-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  HZ-1116. 68666-2-PBS was HRP conjugated as the detection antibody Range: 62.5-4000 pg/mL.

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  Sample test of MP50006-1

  Serum of sixteen individual healthy human donors was measured. Fourteen measured less than the lowest human IL-17F standard, 62.5 pg/mL. Two samples read 46.2 pg/mL and 277.7 pg/mL.

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  Sample test of MP50006-1

  Human peripheral blood mononuclear cells (PBMC) was cultured in RPMI 1640 supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, and 100 μg/mL of streptomycin sulfate. The cells were unstimulated or stimulated with 1 μg/mL CD3 for 4 days. An aliquot of the cell culture supernatant was removed, the mean IL-17F concentration was undetectable in unstimulated PBMC supernatant, 3,282.8 pg/mL in CD3 stimulated PBMC supernatant.

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Informations sur le produit

68666-2-PBS cible IL-17F dans les applications de Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-17F Protéine recombinante HZ-1116
• Nom complet: interleukin 17F
• Symbole du gène: IL-17F
• Identification du gène (NCBI): 112744
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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