Anticorps Recombinant de lapin anti-IL-1R1

IL-1R1 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241392G7

N° de cat : 84094-6-PBS

Synonymes

IL-1R-1, Il1r1, EC:3.2.2.6, CD121a, CD121 antigen-like family member A

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Galerie de données de validation

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  WB analysis of mouse liver using 84094-6-RR (same clone as 84094-6-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 84094-6-RR (IL-1R1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84094-6-PBS in a different storage buffer formulation.

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  WB analysis of EL-4-B5 using 84094-6-RR (same clone as 84094-6-PBS)

  EL-4-B5 cells were subjected to SDS PAGE followed by western blot with 84094-6-RR (IL-1R1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84094-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84094-6-RR (same clone as 84094-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84094-6-RR (IL-1R1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84094-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84094-6-RR (same clone as 84094-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84094-6-RR (IL-1R1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84094-6-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 84094-6-RR (same clone as 84094-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using IL-1R1 antibody (84094-6-RR, Clone: 241392G7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84094-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84094-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84094-6-RR against Mouse IL-1R1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84094-6-PBS cible IL-1R1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 1 receptor, type I
• Masse moléculaire calculée: 67 kDa
• Poids moléculaire observé: 75-100 kDa
• Numéro d’acquisition GenBank: NM_008362.2
• Symbole du gène: Il1r1
• Identification du gène (NCBI): 16177
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IL-1R1 (IL-1 receptor type 1, also known as p80, IL-1R-alpha or CD121a) is a 80 kDa transmembrane glycoprotein and a member of the interleukin-1 receptor family (PMID: 35163653; 29248002). It is a receptor for IL-1α, IL-1β, and interleukin-1 receptor antagonist (IL-1Ra). IL-1α and IL-1β interact with the extracellular domain of IL-1R1, triggering the recruitment of an accessory receptor, the IL-1RAcP, resulting in a functional receptor complex that initiates IL-1R1 signaling cascades (PMID: 35163653). IL-1Ra competes with active IL-1 and blocks binding to their common activating receptor IL-1R1 and, thus, inhibits IL-1 signaling (PMID: 35332327; 11641511). It has been reported that IL-1R1 may also bind IL-38 (PMID: 29248002). IL-1R1 expression has been found in many cell types including T cells, macrophages, neutrophils, eosinophils, basophils, mast cells, fibroblasts, and endothelial cells. IL-1R1 can be released from the cell surface following proteolytic cleavage, generating a soluble form of 55-60 kDa (PMID: 17324958).

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