Anticorps Recombinant de lapin anti-IL-1RA

IL-1RA Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241879B3

N° de cat : 84532-4-RR

Synonymes

IL 1ra, IL1RN, IL1RA, IL 1RN, ICIL-1RA

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Galerie de données de validation

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  WB analysis using 84532-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84532-4-RR (IL-1RA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84532-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84532-4-RR against Human IL-1RA were performed. The affinity constant is 0.302 nM.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HepG2, cellules THP-1

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84532-4-RR cible IL-1RA dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IL-1RA Protéine recombinante Ag1277
• Nom complet: interleukin 1 receptor antagonist
• Masse moléculaire calculée: 20 kDa
• Poids moléculaire observé: 16-18 kDa
• Numéro d’acquisition GenBank: BC009745
• Symbole du gène: IL-1RA
• Identification du gène (NCBI): 3557
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-1 receptor antagonist (IL-1RA) is a critical member of the IL-1 family of cytokines, which plays a significant role in modulating inflammatory responses. IL-1RA exists in two main forms: a secreted form (sIL-1Ra) and an intracellular form (icIL-1Ra). The secreted form is produced by various cells including monocytes, macrophages, neutrophils, and other cells, while the intracellular form is found in keratinocytes and other epithelial cells, as well as in monocytes and fibroblasts. IL-1RA is important in host defense against endotoxin-induced injury and is produced in numerous experimental animal models of disease, as well as in human autoimmune and chronic inflammatory diseases. It acts as an anti-inflammatory agent by maintaining the balance between pro-inflammatory IL-1α/β and its own anti-inflammatory effects.

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