Anticorps Recombinant de lapin anti-IL-1RA

IL-1RA  Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240208G5

N° de cat : 83335-4-PBS

Synonymes

Il1rn, IL-1RN, 240208G5, IL 1ra, IL-1RA

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Galerie de données de validation

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  WB analysis of mouse skin using 83335-4-RR (same clone as 83335-4-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 83335-4-RR (IL-1RN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83335-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skin using 83335-4-RR (same clone as 83335-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83335-4-RR (IL-1RN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83335-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skin using 83335-4-RR (same clone as 83335-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83335-4-RR (IL-1RN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83335-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00353-2

  Cytometric bead array standard curve of MP00353-2, MOUSE IL-1RA Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83335-4-PBS. Detection antibody: 83335-3-PBS. Standard: Eg0685. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83335-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83335-4-RR against Mouse IL-1RN were performed. The affinity constant is 1.75 nM.

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Informations sur le produit

83335-4-PBS cible IL-1RA  dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IL-1RA Protéine recombinante Eg0685
• Nom complet: interleukin 1 receptor antagonist
• Masse moléculaire calculée: 18KD
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: NM_031167.5
• Symbole du gène: IL-1RA
• Identification du gène (NCBI): 16181
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-1 receptor antagonist (IL-1RA) is a critical member of the IL-1 family of cytokines, which plays a significant role in modulating inflammatory responses. IL-1RA exists in two main forms: a secreted form (sIL-1Ra) and an intracellular form (icIL-1Ra). The secreted form is produced by various cells including monocytes, macrophages, neutrophils, and other cells, while the intracellular form is found in keratinocytes and other epithelial cells, as well as in monocytes and fibroblasts. IL-1RA is important in host defense against endotoxin-induced injury and is produced in numerous experimental animal models of disease, as well as in human autoimmune and chronic inflammatory diseases. It acts as an anti-inflammatory agent by maintaining the balance between pro-inflammatory IL-1α/β and its own anti-inflammatory effects.

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