Anticorps Recombinant de lapin anti-IL-1RA

IL-1RA  Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

240208G5

N° de cat : 83335-4-RR

Synonymes

IL-1RN, Il1rn, Interleukin-1, IL-1RA, IL 1ra

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Galerie de données de validation

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  WB analysis of mouse skin using 83335-4-RR

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 83335-4-RR (IL-1RN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skin using 83335-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83335-4-RR (IL-1RN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 83335-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83335-4-RR (IL-1RN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83335-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83335-4-RR against Mouse IL-1RN were performed. The affinity constant is 1.75 nM.

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Applications testées

• Résultats positifs en WB: tissu cutané de souris,
• Résultats positifs en IHC: tissu cutané de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83335-4-RR cible IL-1RA  dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 1 receptor antagonist
• Masse moléculaire calculée: 18KD
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: NM_031167.5
• Symbole du gène: IL-1RA
• Identification du gène (NCBI): 16181
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-1 receptor antagonist (IL-1RA) is a critical member of the IL-1 family of cytokines, which plays a significant role in modulating inflammatory responses. IL-1RA exists in two main forms: a secreted form (sIL-1Ra) and an intracellular form (icIL-1Ra). The secreted form is produced by various cells including monocytes, macrophages, neutrophils, and other cells, while the intracellular form is found in keratinocytes and other epithelial cells, as well as in monocytes and fibroblasts. IL-1RA is important in host defense against endotoxin-induced injury and is produced in numerous experimental animal models of disease, as well as in human autoimmune and chronic inflammatory diseases. It acts as an anti-inflammatory agent by maintaining the balance between pro-inflammatory IL-1α/β and its own anti-inflammatory effects.

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