Anticorps Recombinant de lapin anti-IL-1 alpha

IL-1 alpha Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

240431B5

N° de cat : 83644-1-RR

Synonymes

IL-1alpha, Il1a, IL1-alpha, IL-1,IL1, IL1 alpha

---

Galerie de données de validation

• 

  WB analysis of RAW 264.7 using 83644-1-RR

  Untreated and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83644-1-RR (IL-1alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of RAW 264.7 using 83644-1-RR

  Immunofluorescent analysis of (4% PFA) fixed LPS treated RAW 264.7 cells using IL-1alpha antibody (83644-1-RR, Clone: 240431B5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 83644-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83644-1-RR against Mouse IL-1alpha were performed. The affinity constant is 1.15 nM.

---

Applications testées

• Résultats positifs en WB: cellules RAW 264.7,
• Résultats positifs en IF/ICC: cellules RAW 264.7 traitées au LPS,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

83644-1-RR cible IL-1 alpha dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 1 alpha
• Masse moléculaire calculée: 31kd
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: NM_010554
• Symbole du gène: Il1a
• Identification du gène (NCBI): 16175
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-1α (IL-1 Alpha) is a protein that plays a crucial role in the regulation of a number of key cellular processes. IL-1 Alpha is a cytokine and a potent mediator of body's response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that IL-1 Alpha also has a role in wound healing, rheumatoid arthritis, Alzheimer's disease and tumor growth. IL-1 Alpha functions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. IL-1 Alpha was found to affect the cell cycle of osteosarcoma cell to reduce cell growth. IL-1 Alpha is initially translated as approximately 30-kD polypeptides that are processed to approximately 17.5-kD polypeptides prior to, or during, release from macrophages.

Publications

Species	Application	Title
mouse	WB	ACS Infect Dis; Rv2741 Promotes Mycobacterium Survival by Modulating Macrophage Function via the IL-1α-MAPK Axis; Authors - Xintong He

{{ptg:RelatedPrimaryAntibodies}}