Anticorps Recombinant de lapin anti-IL-2

IL-2 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240416H3

N° de cat : 83558-5-RR

Synonymes

IL2, TCGF, Interleukin-2, interleukin 2, IL 2

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Galerie de données de validation

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  WB analysis using 83558-5-RR

  Untreated, PMA, A23187 and Brefeldin A treated Jurkat cells were subjected to SDS PAGE followed by western blot with 83558-5-RR (IL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83558-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83558-5-RR against Human IL-2 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83558-5-RR cible IL-2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 2
• Masse moléculaire calculée: 153 aa, 18 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC070338
• Symbole du gène: IL2
• Identification du gène (NCBI): 3558
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IL2 is a secreted cytokine involved in the operation and regulation of immune system. IL2 plays a pivotal role in T-cell activation and effector functions, including T cell proliferation, interferon-γproduction and cytotoxicity. IL2 can stimulate the propagation of lymphocytes and induce the cytotoxic T lymphocytes and lymphokine-activated killer cells for tumor cells. IL2 also influences memory T cell homeostasis through the regulation of memory T cell numbers and enhances major histocompatibility complex II (MHC II) expression on tumor cells.

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