Anticorps Monoclonal anti-IL-28A

IL-28A Monoclonal Antibody for IHC, IF-P, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IHC, IF-P, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

3B4A1

N° de cat : 60270-1-PBS

Synonymes

IL28A, Cytokine Zcyto20, IFN lambda 2, IFNL2, IFN-lambda-2

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP50008-1

  Sandwich ELISA standard curve of MP50008-1, IL-28A Monoclonal Matched Antibody Pair - PBS only. 60270-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  HZ-1235. 68669-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

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  Sample test of MP50008-1

  A549 cells were cultured unstimulated or stimulated with 10 μg/mL Poly I:C and 5 μg/mL Lip for 1 day. The mean IL-28A concentration was undetectable in unstimulated supernatant, 3,774.2 pg/mL in stimulated supernatant.

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  Sample test of MP50008-1

  HUVEC cells were cultured unstimulated or stimulated with 100 ng/mL IFN-a and 10 μg/mL Poly I:C for 3 hours. The mean IL-28A concentration was undetectable in unstimulated supernatant, 70.0 pg/mL in stimulated supernatant.

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  IHC staining of human tonsillitis using 60270-1-Ig (same clone as 60270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60270-1-Ig (IL-28A antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60270-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60270-1-Ig (same clone as 60270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60270-1-Ig (IL-28A antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60270-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 60270-1-Ig (same clone as 60270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 60270-1-Ig (IL-28A antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60270-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 60270-1-Ig (same clone as 60270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 60270-1-Ig (IL-28A antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60270-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 60270-1-Ig (same clone as 60270-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-28A antibody (60270-1-Ig, Clone: 3B4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60270-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 60270-1-Ig (same clone as 60270-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-28A antibody (60270-1-Ig, Clone: 3B4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60270-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60270-1-PBS cible IL-28A dans les applications de IHC, IF-P, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-28A Protéine recombinante Ag18525
• Nom complet: interleukin 28A (interferon, lambda 2)
• Masse moléculaire calculée: 200 aa, 22 kDa
• Numéro d’acquisition GenBank: BC113583
• Symbole du gène: IL-28A
• Identification du gène (NCBI): 282616
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IL28A, also named as IFNL2 and ZCYT020, is a cytokine with immunomodulatory activity. It up-regulates MHC class I antigen expression. IL28A is a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. It plays a significant role in the antiviral immune defense in the intestinal epithelium.

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