Anticorps Recombinant de lapin anti-IL-4

IL-4 Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

241249H7

N° de cat : 84422-5-PBS

Synonymes

241249H7, B-cell growth factor 1, B-cell IGG differentiation factor, B-cell stimulatory factor 1, BSF-1

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Galerie de données de validation

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  WB analysis of Recombinant protein using 84422-5-RR (same clone as 84422-5-PBS)

  Rat IL-4 recombinant protein (Cat No. Eg113) was subjected to SDS PAGE followed by western blot with 84422-5-RR (IL-4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84422-5-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01293-4

  Sandwich ELISA standard curve of MP01293-4, Rat IL-4 Recombinant Matched Antibody Pair - PBS only. 84422-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1113. 84422-5-PBS was Biotin conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

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  Sample test of MP01293-4

  Serum of eight individual healthy human donors was measured. Seven measured less than the lowest rat IL-4 standard, 39.1 pg/mL. One sample read 18.7 pg/mL.

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  Sample test of MP01293-4

  One rat thymus was cultured for 18 hours in RPMI 1640 supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, and 100 μg/mL of streptomycin sulfate. The cells were stimulated with 5 μg/mL of PHA and 50 ng/mL of PMA for 7 days. An aliquot of the cell culture supernatant was removed, assayed for rat IL-4 and measured 98.5 pg/mL.

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Informations sur le produit

84422-5-PBS cible IL-4 dans les applications de WB, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IL-4 Protéine recombinante Eg1113
• Nom complet: interleukin 4
• Masse moléculaire calculée: 16kDa
• Numéro d’acquisition GenBank: NM_201270.1
• Symbole du gène: IL-4
• Identification du gène (NCBI): 287287
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-4 (IL-4) is a pleiotropic cytokine that plays a crucial role in regulating immune responses and mediating type-2 immune reactions. It is primarily produced by T-helper 2 (Th2) cells, mast cells, eosinophils, basophils, and other immune cells. IL-4 exerts its effects by binding to type I and type II receptors, initiating a signaling cascade that involves the phosphorylation of STAT6 and the transcription of IL-4-responsive genes. This pathway is essential for the regulation of immune cell functions and the maintenance of immune homeostasis.

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