Anticorps Recombinant de lapin anti-IL-4RA/CD124

IL-4RA/CD124 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242262E3

N° de cat : 84758-5-PBS

Synonymes

242262E3, CD124, IL-4 receptor subunit alpha, Il4r, IL-4R subunit alpha

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 84758-5-RR (same clone as 84758-5-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84758-5-RR (IL-4 R alpha/CD124 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84758-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 84758-5-RR (same clone as 84758-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84758-5-RR (IL-4RA/CD124 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84758-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 84758-5-RR (same clone as 84758-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84758-5-RR (IL-4RA/CD124 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84758-5-PBS in a different storage buffer formulation.

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  IHC staining of human tonsil using 84758-5-RR (same clone as 84758-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 84758-5-RR (IL-4RA/CD124 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84758-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsil using 84758-5-RR (same clone as 84758-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 84758-5-RR (IL-4RA/CD124 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84758-5-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 84758-5-RR (same clone as 84758-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84758-5-RR (IL-4RA/CD124 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84758-5-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 84758-5-RR (same clone as 84758-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84758-5-RR (IL-4RA/CD124 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84758-5-PBS in a different storage buffer formulation.

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Informations sur le produit

84758-5-PBS cible IL-4RA/CD124 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IL-4RA/CD124 Protéine recombinante Eg2176
• Nom complet: interleukin 4 receptor, alpha
• Masse moléculaire calculée: 88 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: NM_001008700.3
• Symbole du gène: Il4ra
• Identification du gène (NCBI): 16190
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IL-4 receptor alpha chain (IL-4RA), also known as CD124, is a transmembrane glycoprotein expressed on a wide variety of hematopoietic and non-hematopoietic cells. It is a receptor for both IL-4 and IL-13. IL-4RA can complex with the common gamma chain (IL-2R gamma or CD132) and utilizes JAK1 and JAK2 for signal transduction, while complexes of IL-4RA with IL-13RA1 subunit mediate signals via JAK2 and Tyk2. The IL-4 response is involved in promoting Th2 differentiation. The IL-4/IL-13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation.

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