Anticorps Recombinant de lapin anti-IL-4RA/CD124

IL-4RA/CD124 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

242262E3

N° de cat : 84758-5-RR

Synonymes

242262E3, CD124, IL-4 receptor subunit alpha, Il4r, IL-4R subunit alpha

---

Galerie de données de validation

• 

  WB analysis of NIH/3T3 using 84758-5-RR

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84758-5-RR (IL-4 R alpha/CD124 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse spleen using 84758-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84758-5-RR (IL-4RA/CD124 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 84758-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84758-5-RR (IL-4RA/CD124 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsil using 84758-5-RR

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 84758-5-RR (IL-4RA/CD124 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsil using 84758-5-RR

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 84758-5-RR (IL-4RA/CD124 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 84758-5-RR

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84758-5-RR (IL-4RA/CD124 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 84758-5-RR

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84758-5-RR (IL-4RA/CD124 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules NIH/3T3,
• Résultats positifs en IHC: tissu amygdalien humain, tissu splénique de rat, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84758-5-RR cible IL-4RA/CD124 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IL-4RA/CD124 Protéine recombinante Eg2176
• Nom complet: interleukin 4 receptor, alpha
• Masse moléculaire calculée: 88 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: NM_001008700.3
• Symbole du gène: Il4ra
• Identification du gène (NCBI): 16190
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IL-4 receptor alpha chain (IL-4RA), also known as CD124, is a transmembrane glycoprotein expressed on a wide variety of hematopoietic and non-hematopoietic cells. It is a receptor for both IL-4 and IL-13. IL-4RA can complex with the common gamma chain (IL-2R gamma or CD132) and utilizes JAK1 and JAK2 for signal transduction, while complexes of IL-4RA with IL-13RA1 subunit mediate signals via JAK2 and Tyk2. The IL-4 response is involved in promoting Th2 differentiation. The IL-4/IL-13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation.

{{ptg:RelatedPrimaryAntibodies}}