Anticorps Recombinant de lapin anti-IL-4RA/CD124

IL-4RA/CD124 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

241824D8

N° de cat : 98203-1-PBS

Synonymes

IL4R, IL 4R subunit alpha, IL 4R alpha, IL 4 receptor subunit alpha, IL 4 binding protein

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Galerie de données de validation

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  FC experiment of human PBMCs using 98203-1-RR (same clone as 98203-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL-4RA/CD124 Rabbit Recombinant Antibody (98203-1-RR, Clone:241824D8) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98203-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98203-1-RR (same clone as 98203-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL-4RA/CD124 Rabbit Recombinant Antibody (98203-1-RR, Clone:241824D8) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98203-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98203-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98203-1-RR against Human IL-4RA/CD124 were performed. The affinity constant is 53.6 pM.

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Informations sur le produit

98203-1-PBS cible IL-4RA/CD124 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 4 receptor
• Masse moléculaire calculée: 90 kDa
• Numéro d’acquisition GenBank: NM_000418.4
• Symbole du gène: IL-4R
• Identification du gène (NCBI): 3566
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

IL-4 receptor alpha chain (IL-4RA), also known as CD124, is a transmembrane glycoprotein expressed on a wide variety of hematopoietic and non-hematopoietic cells. It is a receptor for both IL-4 and IL-13. IL-4RA can complex with the common gamma chain (IL-2R gamma or CD132) and utilizes JAK1 and JAK2 for signal transduction, while complexes of IL-4RA with IL-13RA1 subunit mediate signals via JAK2 and Tyk2. The IL-4 response is involved in promoting Th2 differentiation. The IL-4/IL-13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation.

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