Anticorps Recombinant de lapin anti-IL-5

IL-5 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

242075A7

N° de cat : 98352-1-PBS

Synonymes

Il5, Il 5, interleukin 5, interleukin-5

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Galerie de données de validation

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  FC experiment of C57BL/6 Th2-polarized splenocytes using 98352-1-RR (same clone as 98352-1-PBS)

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated C57BL/6 Th2-polarized splenocytes were intracellularly stained with 0.125 ug Anti-Mouse IL5 Rabbit RecAb (98352-1-RR, Clone:242075A7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD4 (GK1.5) (APC-65104, Clone: GK1.5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98352-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98352-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98352-1-RR against Mouse IL-5 were performed. The affinity constant is 5.79 nM.

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Informations sur le produit

98352-1-PBS cible IL-5 dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 5
• Masse moléculaire calculée: 15 aa
• Numéro d’acquisition GenBank: NM_010558
• Symbole du gène: Il5
• Identification du gène (NCBI): 16191
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

Interleukin-5 (IL-5) is a critical cytokine that regulates the growth, activation, and survival of eosinophils. IL-5 is produced by both hematopoietic and non-hematopoietic cells including T cells, granulocytes, and natural helper cells. IL-5 exerts its effects for proliferation and differentiation via receptors that comprise an IL-5-specific α and common β-subunit. Overexpression of IL-5 in vivo significantly increases eosinophils and B cells in number, while mice lacking a functional gene for IL-5 or IL-5 receptor display a number of developmental and functional impairments in B cells and eosinophil lineages.

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