Anticorps Monoclonal anti-IL-6

IL-6 Monoclonal Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

6C12C4

N° de cat : 68659-1-PBS

Synonymes

IL6, B-cell stimulatory factor 2, IFN-beta-2, IL 6, Interferon beta-2

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Galerie de données de validation

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  Sandwich ELISA sample test of MP50032-1

  Serum of sixteen individual healthy human donors was measured. The IL-6 concentration of detected samples was determined to be 73.8 pg/mL with a range of ND-113.9 pg/mL.

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  Sandwich ELISA sample test of MP50032-1

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 1 day or 3 days. For the PBMC supernatant cultured for 1 day, the mean IL-6 concentration was determined to be 0.1 ng/mL in unstimulated PBMC supernatant, 19.0 ng/mL in PHA stimulated PBMC supernatant. For the PBMC supernatant cultured for 3 days, the mean IL-6 concentration was determined to be 0.1 ng/mL in unstimulated PBMC supernatant, 39.0 ng/mL in PHA stimulated PBMC supernatant.

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  Sandwich ELISA standard curve of MP50032-1

  Sandwich ELISA standard curve of MP50032-1, IL6 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68659-1-PBS. Detection antibody: 68659-2-PBS. Standard: HZ-1019. Range: 7.8-500 pg/mL.

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  Cytometric bead array sample test of MP50032-1

  Cytometric bead array of HT-1080 cells supernatant using MP50032-1, IL-6 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68659-1-PBS. Detection antibody: 68659-2-PBS. HT-1080 cells were inoculated into 6 well plate at 40000 cells/well followed by culture for 6 days in normal culture media or IL-17A (Cat.NO. HZ-1113, 40 ng/mL) containing media. 20 μL of the supernatant from day 1, 2, 3 and day 6 was analyzed using MP50032-1 (IL-6 Monoclonal Matched Antibody Pair). The signal (Median Fluorescenence Intensity) is highly correlated with the stimulation time.Non-related target B2M Monoclonal Matched Antibody Pair (MP50108-1) was served as control.

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  Cytometric bead array standard curve of MP50032-1

  Cytometric bead array standard curve of MP50032-1, IL-6 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68659-1-PBS. Detection antibody: 68659-2-PBS. Standard:HZ-1019. Range: 0.098-12.5 ng/mL.

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Informations sur le produit

68659-1-PBS cible IL-6 dans les applications de Cytometric bead array, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-6 Protéine recombinante HZ-1019
• Nom complet: interleukin 6 (interferon, beta 2)
• Symbole du gène: IL6
• Identification du gène (NCBI): 3569
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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