Anticorps Recombinant de lapin anti-IL-6

IL-6 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

240701B9

N° de cat : 83795-7-RR

Synonymes

Il6, Il 6, interleukin 6, interleukin-6

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Galerie de données de validation

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  WB analysis of RAW 264.7 using 83795-7-RR

  PMA, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83795-7-RR (IL-6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse spleen using 83795-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83795-7-RR (IL-6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 83795-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83795-7-RR (IL-6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 83795-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83795-7-RR against Mouse IL-6 were performed. The affinity constant is 29.2 nM.

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Applications testées

• Résultats positifs en WB: PMA, LPS and Brefeldin A treated RAW 264.7 cells,
• Résultats positifs en IHC: tissu splénique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83795-7-RR cible IL-6 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IL-6 Protéine recombinante Eg0973
• Nom complet: interleukin 6
• Masse moléculaire calculée: 24 kDa
• Numéro d’acquisition GenBank: NM_031168
• Symbole du gène: Il6
• Identification du gène (NCBI): 16193
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

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