Anticorps Recombinant de lapin anti-IL-6

IL-6 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

240670D12

N° de cat : 98087-1-PBS

Synonymes

IL6, Interferon beta-2, IL 6, IFN-beta-2, B-cell stimulatory factor 2

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Galerie de données de validation

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  FC experiment of human PBMCs using 98087-1-RR (same clone as 98087-1-PBS)

  1x10^6 untreated or LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-6 Rabbit Recombinant Antibody (98087-1-RR, Clone:240670D12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated. This data was developed using the same antibody clone with 98087-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98087-1-RR (same clone as 98087-1-PBS)

  1x10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-6 Rabbit Recombinant Antibody (98087-1-RR, Clone:240670D12) (red) or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red). 1x10^6 untreated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL6 Rabbit Recombinant Antibody (98087-1-RR, Clone:240670D12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated. This data was developed using the same antibody clone with 98087-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98087-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98087-1-RR against Human IL-6 were performed. The affinity constant is 0.322 nM.

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Informations sur le produit

98087-1-PBS cible IL-6 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 6 (interferon, beta 2)
• Masse moléculaire calculée: 212 aa, 24 kDa
• Numéro d’acquisition GenBank: BC015511
• Symbole du gène: IL6
• Identification du gène (NCBI): 3569
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

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