Anticorps Recombinant de lapin anti-CD126/IL-6R alpha

CD126/IL-6R alpha Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240734D1

N° de cat : 83688-4-RR

Synonymes

CD126, IL-6R alpha, 240734D1, IL 6R alpha, IL 6R 1

---

Galerie de données de validation

• 

  WB analysis of RAW 264.7 using 83688-4-RR

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83688-4-RR (CD126/IL-6R alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 83688-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83688-4-RR against Human CD126/IL-6R alpha were performed. The affinity constant is 0.80 nM.

---

Applications testées

• Résultats positifs en WB: cellules RAW 264.7,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83688-4-RR cible CD126/IL-6R alpha dans les applications de WB, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 6 receptor
• Masse moléculaire calculée: 468 aa, 52 kDa
• Poids moléculaire observé: 65 kDA
• Numéro d’acquisition GenBank: BC132684
• Symbole du gène: IL-6R alpha
• Identification du gène (NCBI): 3570
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R, the complex of IL-6 and IL-6R associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while IL-6R is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892).

{{ptg:RelatedPrimaryAntibodies}}