Anticorps Monoclonal anti-IL-7

IL-7 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

FC

Conjugaison

FITC Plus Fluorescent Dye

CloneNo.

1C11B11

N° de cat : FITC-65281

Synonymes

IL 7, IL7, interleukin 7

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Galerie de données de validation

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  FC experiment of human PBMCs using FITC-65281

  1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug FITC Plus Anti-Human IL-7 (CL488-65281, Clone:1C11B11) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65281

  1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug FITC Plus Anti-Human IL-7 (FITC-65281, Clone:1C11B11) (red) or Mouse IgG1 Isotype Control (blue), or unactivated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug FITC Plus Anti-Human IL-7 (FITC-65281, Clone:1C11B11) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: LPS and Brefeldin A treated human PBMCs,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-65281 cible IL-7 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-7 Protéine recombinante HZ-1281
• Nom complet: interleukin 7
• Masse moléculaire calculée: 20 kDa
• Numéro d’acquisition GenBank: BC047698
• Symbole du gène: IL7
• Identification du gène (NCBI): 3574
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Interleukin-7 (IL-7) is a cytokine involved in B and T cell development. It plays an active role in the development, survival, maintaining and restoring homeostasis of mature T lymphocytes and is a key regulator of the commitment, survival, proliferation and maturation of B cells during development. Furthermore, IL-7 can improve the antiviral function and expansion of natural killer (NK) cells and regulate the development and differentiation of dendritic cells. IL-7 has also been reported as a regulator of the development of central nervous system and myogenesis and skeletal muscle cell migration. (PMID: 29663382; 29655570; 29449560)